Ribosome Structure and Function 2022

Sun. 10

Mon. 11

Tue. 12

Wed. 13

Thu. 14

09:00

10:00

11:00

12:00

13:00

14:00

15:00

16:00

17:00

18:00

19:00

20:00

21:00

22:00

Registration & Welcome Drinks

16:00 - 17:40 (1h40)

Registration & Welcome Drinks

Registration at the front desk of the Palais de la Bourse (19, Place de la Bourse, 33000 Bordeaux)

Opening remarks

17:40 - 17:55 (15min)

Opening remarks

Main hall (Espace de la Bourse)

Keynote Lecture I

17:55 - 18:40 (45min)

Keynote Lecture I

Main hall (Espace de la Bourse)

Jody Puglisi

Ribosome biogenesis and proto-ribosomes

18:40 - 20:00 (1h20)

Ribosome biogenesis and proto-ribosomes

Main hall (Espace de la Bourse)

› A cold look at the formation and activation of the bacterial large ribosomal subunit - Christian Spahn 18:40-19:00 (20min)

› Early Intermediates in 50S ribosome assembly - James Williamson 19:00-19:20 (20min)

› Translationally active proto-ribosomes with smaller rRNA - Jessica Willi 19:20-19:40 (20min)

› The proto-ribosome as the kernel of origin of life - Ada Yonath 19:40-20:00 (20min)

Initiation

9:00 - 10:40 (1h40)

Initiation

Main hall (Espace de la Bourse)

› Dissecting ribosome and translation initiation factor binding using high throughput approaches reveals novel regulatory elements in 5′-UTRs - Ritam Neupane 09:00-09:20 (20min)

› GTP hydrolysis by eIF2 and binding of eIF5B rearrange the human 48S initiation complex for 60S subunit joining - Niels Fischer 09:20-09:40 (20min)

› Structure of a human 48S reveals how eIF4F and eIF4A regulate translation initiation - Jailson Brito Querido 09:40-10:00 (20min)

› The structural dynamics of mRNA recruitment during eukaryotic translation initiation - Riley Gentry 10:00-10:20 (20min)

› Compact IF2 gates the ribosome to elongation - Matthieu Gagnon 10:20-10:40 (20min)

Coffee break

10:40 - 11:20 (40min)

Coffee break

Main hall (Espace de la Bourse)

Decoding and elongation I

11:20 - 12:40 (1h20)

Decoding and elongation I

Main hall (Espace de la Bourse)

› Geometric alignment of aminoacyl-tRNA relative to catalytic centers of the ribosome underpins accurate mRNA decoding - Karissa Sanbonmatsu 11:20-11:40 (20min)

› Structural insights into the decoding capability of tRNA with cytidine modifications - Naho Akiyama 11:40-12:00 (20min)

› Codon position-specific engineering of translation kinetics monitored by Live Imaging on Single Cell Arrays (LISCA) - Sophia Rudorf 12:00-12:20 (20min)

› Impact of ribosomes, mRNA sequence and nascent protein chain on efficiency and accuracy of protein synthesis - Sergej Djuranovic 12:20-12:40 (20min)

Lunch

12:40 - 14:00 (1h20)

Lunch

Main hall (Espace de la Bourse)

Decoding and elongation II

14:00 - 16:00 (2h)

Decoding and elongation II

Main hall (Espace de la Bourse)

› Specific length and structure rather than high thermodynamic stability enable regulatory mRNA stem-loops to pause translation - Dmitri Ermolenko 14:00-14:20 (20min)

› Insights into the ribosome function from the structures of non-arrested ribosome nascent chain complexes. - Yury Polikanov 14:20-14:40 (20min)

› Variable sequences in the Archaeal A loop stabilize the bacterial ribosome - Amos Nissley 14:40-15:00 (20min)

› Revealing transient processes governing translation through the integration of single-molecule imaging and cryo-electron microscopy - Scott Blanchard 15:00-15:20 (20min)

› Ribosome stalling in Huntington disease - Srinivasa Subramaniam 15:20-15:40 (20min)

› Alexander Spirin (1931-2020). Role of diphthamide in the ribosomal translocation. E-site specificity of Candida albicans ribosome. - Marat Yusupov 15:40-16:00 (20min)

Coffee break

16:00 - 16:40 (40min)

Coffee break

Main hall (Espace de la Bourse)

Disrupted elongation and termination

16:40 - 18:20 (1h40)

Disrupted elongation and termination

Main hall (Espace de la Bourse)

› Nascent peptide-induced translation discontinuation in eukaryotes impacts biased amino acid usage in proteomes. - Yuhei Chadani 16:40-17:00 (20min)

› How the ribosome shifts to the +1-frame - Ya-Ming Hou 17:00-17:20 (20min)

› Start codon-associated ribosomal frameshifting mediates nutrient stress adaptation - Shu-Bing Qian 17:20-17:40 (20min)

› Single-molecule imaging in cells reveals the dynamics of programmed stop-codon readthrough by ribosomes - Nicholas Guydosh 17:40-18:00 (20min)

› New results on dynamic processes in eubacterial and eukaryotic translation from time-resolved cryo-EM - Joachim Frank 18:00-18:20 (20min)

Drinks & finger food | Poster Session I

18:20 - 20:30 (2h10)

Drinks & finger food | Poster Session I

Main hall (Espace de la Bourse)

Co-translational folding and targeting

9:00 - 10:40 (1h40)

Co-translational folding and targeting

Main hall (Espace de la Bourse)

› The role of NAC in SRP mediated protein targeting to the ER - Nenad Ban 09:00-09:20 (20min)

› AP Profiling: Resolving co-translational protein folding pathways in vivo - Christian Kaiser 09:20-09:40 (20min)

› Cotranslational folding of cytosolic and membrane proteins - Marina Rodnina 09:40-10:00 (20min)

› Structural biology of protein folding on the ribosome - John Christodoulou 10:00-10:20 (20min)

› Structural studies of protein folding and misfolding during biogenesis on the ribosome - Lisa Cabrita 10:20-10:40 (20min)

Coffee break

10:40 - 11:20 (40min)

Coffee break

Main hall (Espace de la Bourse)

Stress response

11:20 - 12:20 (1h)

Stress response

Main hall (Espace de la Bourse)

› (p)ppGpp controls stringent factors by exploiting antagonistic allosteric coupling between catalytic domains - Vasili Hauryliuk 11:20-11:40 (20min)

› RelA-SpoT Homolog toxins pyrophosphorylate the CCA end of tRNA to inhibit protein synthesis - Tatsuaki Kurata 11:40-12:00 (20min)

› Structural insights into ribosomal triggering of stress responses. - Andrei Korostelev 12:00-12:20 (20min)

Methods

12:20 - 13:00 (40min)

Methods

Main hall (Espace de la Bourse)

› Effects of cryo-EM cooling on structural ensembles - Helmut Grubmueller 12:20-12:40 (20min)

› Single-molecule tracking for kinetics measurements of protein synthesis, folding, and targeting inside living cells - Mikhail Metelev 12:40-13:00 (20min)

Box lunch

13:00 - 14:00 (1h)

Box lunch

Free time

14:00 - 18:00 (4h)

Free time

Optional tours of Bordeaux Center, Arcachon or Saint-Emilion (separate registration required)

Quality control in translation I

9:00 - 10:40 (1h40)

Quality control in translation I

Main hall (Espace de la Bourse)

› An E3 ligase network engages GCN1 to promote elongation factor-1a degradation on stalled ribosomes - Jack Taunton 09:00-09:20 (20min)

› Mechanism of ribosome-associated mRNA degradation in the tubulin autoregulation pathway - Markus Höpfler 09:20-09:40 (20min)

› Mechanistic insights into ribosomal protein degradation - Sichen Shao 09:40-10:00 (20min)

› Ribosome collisions induce mRNA cleavage and ribosome rescue in bacteria - Allen Buskirk 10:00-10:20 (20min)

› Ribosome collisions, quality control and stress response - Hani Zaher 10:20-10:40 (20min)

Coffee break

10:40 - 11:20 (40min)

Coffee break

Main hall (Espace de la Bourse)

Quality control in translation II

11:20 - 12:40 (1h20)

Quality control in translation II

Main hall (Espace de la Bourse)

› ROS-induced ribosome pausing underlies ZAK-alpha-mediated metabolic decline in obesity and aging - Simon Bekker-Jensen 11:20-11:40 (20min)

› GCN2 activation by ribosome stalling – a sensor of disease-associated alterations in translation dynamics - Zoya Ignatova 11:40-12:00 (20min)

› Sensing and Targeting of Individual Stalled 80S Ribosomes for Non-functional rRNA Turnover - Sihan Li 12:00-12:20 (20min)

› Structural studies of co-translational quality control - Petr Tesina 12:20-12:40 (20min)

Lunch

12:40 - 14:00 (1h20)

Lunch

Main hall (Espace de la Bourse)

Antibiotics and resistance

14:00 - 16:00 (2h)

Antibiotics and resistance

Main hall (Espace de la Bourse)

› Ribosomal antibiotics as modulators of translation - Alexander Mankin 14:00-14:20 (20min)

› Context-dependent inhibition of bacterial protein synthesis by ribosome-targeting antibiotics - Axel Innis 14:20-14:40 (20min)

› Context-Specific Inhibition of Translation by Oxazolidinone Antibiotics - Danica Galonic Fujimori 14:40-15:00 (20min)

› Ribosome-targeting antibiotics and bacterial resistance mechanisms - Daniel Wilson 15:00-15:20 (20min)

› Cryo-EM studies of all four E. coli ABCF paralogs establish the general principles underlying reciprocal allosteric linkage of the ATPase activity of ABCF proteins to global ribosome conformation and PTC structure - Shikha Singh 15:20-15:40 (20min)

› Complementary mechanisms of ribosome inhibition by known antibiotics - Pohl Milon 15:40-16:00 (20min)

Coffee break

16:00 - 16:40 (40min)

Coffee break

Main hall (Espace de la Bourse)

Alternative ribosome forms

16:40 - 18:20 (1h40)

Alternative ribosome forms

Main hall (Espace de la Bourse)

› A human rRNA catalog, the Ribome, associates with development, aging, and cancer - Daphna Rothschild 16:40-17:00 (20min)

› Expanding our knowledge on rRNA functions: expansion segments as modulators of the eukaryal ribosome - Robert Rauscher 17:00-17:20 (20min)

› Evolution of biological novelty through ribosome specialization - Amy Lee 17:20-17:40 (20min)

› Ribosomes lacking bS21 gain function to regulate protein synthesis - Kurt Fredrick 17:40-18:00 (20min)

› Ribosme interactome recomposition by viruses - Thibault Sohier 18:00-18:20 (20min)

Drinks & finger food | Poster Session II

18:20 - 20:30 (2h10)

Drinks & finger food | Poster Session II

Main hall (Espace de la Bourse)

Keynote Lecture II

9:00 - 9:45 (45min)

Keynote Lecture II

Main hall (Espace de la Bourse)

Rachel Green

Ribosomes in organelles and pathogens

9:45 - 11:05 (1h20)

Ribosomes in organelles and pathogens

Main hall (Espace de la Bourse)

› Activation mechanism of mitochondrial translation by LRPPRC-SLIRP - Alexey Amunts 09:45-10:05 (20min)

› Human mitochondria require mtRF1 for translation termination at non-canonical stop codons - Joanna Rorbach 10:05-10:25 (20min)

› The Giardia lamblia ribosome structure at 2.49 Å reveals divergence in several biological pathways and the mode of emetine function - Jeffrey Kieft 10:25-10:45 (20min)

› Translocation Intermediates of Giardia intestinalis Ribosome Provide Insights into the Evolution of Eukaryotic Translation Machinery - Suparna Sanyal 10:45-11:05 (20min)

Coffee break

11:05 - 11:35 (30min)

Coffee break

Main hall (Espace de la Bourse)

Regulation of translation

11:35 - 13:15 (1h40)

Regulation of translation

Main hall (Espace de la Bourse)

› eIF5A is a Sensor and Effector for Polyamine Regulation of Translation - 11:35-11:55 (20min)

› ANTIBIOTIC RESISTANCE OR SIGNALING? The role of ABCF-ATPases in antibiotic producers. - 11:55-12:15 (20min)

› The ABC-F protein EttA regulates specific protein synthesis by alleviating early ribosomal translational stalling during mRNA translation. - 12:15-12:35 (20min)

› A new paradigm in virus-host arms race: How does the host encoded ZAP-S alter conformational dynamics and translation of frameshifting RNAs? - Neva Caliskan 12:35-12:55 (20min)

› Alterations in mRNA translation initiation in colorectal cancer - Yaser Hashem 12:55-13:15 (20min)

Free time

13:15 - 19:00 (5h45)

Free time

Closing Gala

19:00 - 22:30 (3h30)

Closing Gala

Main hall (Espace de la Bourse)